circRNA basic information
circBase ID: hsa_circ_0008274
Name: -
Synonym: circ0008274 / circ00008274
Host Gene: -
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: nucleus and cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer / GC
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

GES-1; TGES-1
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: Argonaute 2
Associated microRNA: miR-526b-5p
Biological function: Inhibits MNNG-induced malignant phenotypes (proliferation, colony formation, migration, invasion, EMT, stemness) in gastric epithelial cells; exosomal circ0008274 mediates the intervention effect of hesperetin on gastric carcinogenesis.
Molecular mechanism: Acts as a ceRNA/miRNA sponge for miR-526b-5p in an exosome-mediated manner; hesperetin modulates exosomal circ0008274 to attenuate MNNG-induced gastric carcinogenesis.
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); EMT (inhibits); stemness (inhibits); ceRNA regulation (other)
Detected method:
Q
Validation methods:

Bioinformatics Analysis; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot
Clinical significance:

-
Description:

This study identifies exosomal circ0008274 as a key circRNA downregulated in the MNNG-induced malignant transformation model and in TGES-1-derived exosomes. Hesperetin restores/upregulates exosomal circ0008274, which suppresses malignant phenotypes (proliferation, migration/invasion, EMT, and stemness) in recipient GES-1 cells by sponging miR-526b-5p (ceRNA mechanism).
Confidence score:

0.7204
Other information
Title:

Interventional effect of hesperetin on N-methyl-N'-nitro-N-nitrosoguanidine-induced exosomal circ008274 in affecting normal cells to promote gastric carcinogenesis.

Journal: World journal of gastroenterology
Published: 2025
PubMed ID: 40308800
Study type:

biological research
Data availability: GSE89143; GSE83521; Dataset available from the first author at liangzhaofeng@ujs.edu.cn
Code availability: -