| Expression pattern: |
UP |
| Associated gene: |
EIF4A3 |
| Associated microRNA: |
- |
| Biological function: |
promotes proliferation, clonality, migration and invasion; promotes HCC progression and metastasis |
| Molecular mechanism: |
hsa_circ_0005397 interacts with the RNA-binding protein EIF4A3 and its pro-tumor effects can be reversed by EIF4A3 knockdown; EIF4A3 depletion reduces hsa_circ_0005397 expression |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); cell cycle (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Transwell Assay |
| Clinical significance: |
potential diagnostic capability in HCC; associated with tumor size and TNM stage |
| Description: |
hsa_circ_0005397 is up-regulated in hepatocellular carcinoma tissues and cell lines and enhances HCC cell proliferation, colony formation, migration/invasion, and cell-cycle progression. Mechanistically, it interacts with the RNA-binding protein EIF4A3, and EIF4A3 knockdown reduces hsa_circ_0005397 levels and reverses its pro-tumor phenotypes. It shows potential diagnostic value (ROC AUC ~0.88) and correlates with tumor size and TNM stage. |
| Confidence score: |
0.8162 |