| Expression pattern: |
DN |
| Associated gene: |
ISM1, AGO2 |
| Associated microRNA: |
miR-1307 |
| Biological function: |
hsa_circ_0091570 suppresses HCC cell proliferation, migration, invasion, metastasis and tumor growth, and promotes apoptosis; knockdown of hsa_circ_0091570 has the opposite effects. |
| Molecular mechanism: |
hsa_circ_0091570 acts as a ceRNA/miRNA sponge for miR-1307, thereby protecting or up-regulating ISM1 expression and suppressing HCC progression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (inhibits); apoptosis (promotes); cell cycle (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Sanger Sequencing; Clinical Sample Validation; ROC Analysis; Survival Analysis; Transfection; CCK8; EdU Staining; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; RNA Pull-Down; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; FISH / smFISH; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); ELISA |
| Clinical significance: |
Decreased hsa_circ_0091570 expression is associated with pathological staging, high Edmondson Grade and portal vein tumor thrombus in HCC patients; ROC analysis showed potential diagnostic capability; hsa_circ_0091570 was related to overall survival rates and may be a prognostic biomarker and therapeutic target. |
| Description: |
hsa_circ_0091570 is down-regulated in HCC tissues and cell lines and functions as a tumor suppressor. It sponges miR-1307 as a ceRNA to regulate ISM1 expression, thereby inhibiting HCC proliferation, migration, invasion, metastasis and tumor growth while promoting apoptosis. Clinically, reduced hsa_circ_0091570 expression is associated with pathological progression, diagnostic potential and prognosis in HCC patients. |
| Confidence score: |
0.8824 |