circRNA basic information
circBase ID: hsa_circ_0000284
Name: hsa_circ_HIPK3
Synonym: circHIPK3 / bladder cancer-related circular RNA-2 (BCRC-2)
Host Gene: HIPK3
Genomic location(hg19): chr11:33307958-33309057:+
Genomic location(hg38): chr11:33286412-33287511:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0004986
MONDO name: urinary bladder carcinoma
Disease details: bladder cancer / BCa
Disease DO ID:
 4007
Disease MeSH ID:
 -
Disease NCIt ID:
 C4912
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

bladder cancer tissues; paired normal bladder tissues; paired adjacent normal bladder tissues; normal bladder urothelium samples
Cell lines:

T24T; UMUC3; SV-HUC-1; HUVEC
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: HPSE, MMP-9, VEGF
Associated microRNA: miR-558
Biological function: Inhibits bladder cancer cell migration, invasion, angiogenesis, tumor growth, and metastasis.
Molecular mechanism: circHIPK3 acts as a miRNA sponge for miR-558 and suppresses HPSE expression, with downstream reduction of MMP-9 and VEGF.
Biological pathway or process:

ceRNA regulation (other); migration (inhibits); invasion (inhibits); angiogenesis (inhibits); metastasis (inhibits); proliferation (inhibits)
Detected method:
Q
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; RNA-seq; Northern Blot; FISH / smFISH; Clinical Sample Validation; RNA Pull-Down; Bioinformatics Analysis; Transfection; Wound Healing Assay; Transwell Assay; Tube Formation Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry)
Clinical significance:

circHIPK3 down-regulation negatively correlates with bladder cancer grade, invasion, and lymph node metastasis, and the study suggests circHIPK3 as a new therapeutic target.
Description:

circHIPK3/hsa_circ_0000284 is a human HIPK3-derived circRNA that is down-regulated in bladder cancer tissues and cell lines. It suppresses bladder cancer migration, invasion, angiogenesis, tumor growth, and metastasis by sponging miR-558 and reducing HPSE expression and downstream MMP-9/VEGF activity. Clinically, lower circHIPK3 expression is associated with higher grade, invasion, and lymph node metastasis, suggesting potential therapeutic relevance.
Confidence score:

0.8426
Other information
Title:

CircHIPK3 sponges miR-558 to suppress heparanase expression in bladder cancer cells.

Journal: EMBO reports
Published: 2017
PubMed ID: 28794202
Study type:

combined biological and clinical study
Data availability: GSE97239; Dataset EV1; Dataset EV2; Dataset EV3
Code availability: -