| Expression pattern: |
UP |
| Associated gene: |
- |
| Associated microRNA: |
hsa-miR-217, hsa-miR-548b-3p, hsa-miR-769-3p |
| Biological function: |
promotes proliferation, migration, and invasion of breast cancer cells |
| Molecular mechanism: |
Predicted miRNA sponge (ceRNA) via binding hsa-miR-217, hsa-miR-548b-3p and hsa-miR-769-3p; downstream targets predicted by bioinformatics |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (other) |
| Detected method: |
Q
H
|
| Validation methods: |
FISH / smFISH; Clinical Sample Validation; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Survival Analysis; Cohort Study; Bioinformatics Analysis; Western Blot |
| Clinical significance: |
High expression of hsa_circ_0006522 was associated with lymphatic metastasis, worse clinical stage, and shorter overall survival; it can serve as an independent prognostic factor for breast cancer. |
| Description: |
In breast cancer, hsa_circ_0006522 is highly expressed and mainly localized in the cytoplasm. It promotes breast cancer cell proliferation, migration, and invasion in vitro. Clinically, high hsa_circ_0006522 expression is associated with lymph node metastasis and shorter overall survival, and it may act via a predicted miRNA-sponge axis involving hsa-miR-217, hsa-miR-548b-3p, and hsa-miR-769-3p. |
| Confidence score: |
0.8143 |