| Expression pattern: |
UP |
| Associated gene: |
ZNF460, ITGA5, FAK, PI3K, AKT |
| Associated microRNA: |
miR-326 |
| Biological function: |
Promotes TNBC cell proliferation, migration, invasion, EMT, cell cycle progression, inhibits apoptosis, and promotes tumor growth and lung metastasis in vivo. |
| Molecular mechanism: |
ZNF460 promotes circRPPH1 expression; circRPPH1 acts as a ceRNA sponge for miR-326 to relieve repression of ITGA5, leading to activation of FAK/PI3K/AKT signaling. |
| Biological pathway or process: |
PI3K/AKT (promotes); EMT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); cell cycle (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; RNA Pull-Down; ChIP / ChIP-seq; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; In Vivo Animal Model; IHC (Immunohistochemistry); IF (Immunofluorescence); Western Blot; H&E Staining; Survival Analysis; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Upregulation/high expression of circRPPH1 is associated with poor prognosis and can be used for diagnosis and prognosis (ROC AUC=0.705) in TNBC. |
| Description: |
circRPPH1 is upregulated in TNBC and its high expression predicts poor overall survival. Functionally, it promotes proliferation, migration/invasion, EMT, cell-cycle progression and tumor growth/metastasis. Mechanistically, ZNF460 increases circRPPH1 expression, and circRPPH1 sponges miR-326 to upregulate ITGA5 and activate FAK/PI3K/AKT signaling. |
| Confidence score: |
0.8942 |