| Expression pattern: |
UP |
| Associated gene: |
HMGA1, AGO2 |
| Associated microRNA: |
miR-545-3p |
| Biological function: |
promotes high-glucose-induced VSMC proliferation, migration, and invasion |
| Molecular mechanism: |
circLRP6 acts as a miR-545-3p sponge and indirectly regulates HMGA1 expression through the miR-545-3p/HMGA1 axis |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; FISH / smFISH; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Wound Healing Assay; Transwell Assay; Western Blot; IF (Immunofluorescence); Bioinformatics Analysis |
| Clinical significance: |
circLRP6 might be a promising target for the treatment of diabetic-related CVDs |
| Description: |
circLRP6 is up-regulated in human VSMCs exposed to high glucose and promotes HG-induced VSMC proliferation, migration, and invasion. Mechanistically, circLRP6 functions as a miR-545-3p sponge to maintain HMGA1 expression, forming a circLRP6/miR-545-3p/HMGA1 axis that may be relevant to diabetic-related cardiovascular disease. |
| Confidence score: |
0.6591 |