| Expression pattern: |
UP |
| Associated gene: |
PIEZO1, FAK, Akt |
| Associated microRNA: |
miR-139-5p |
| Biological function: |
Promotes GBM cell proliferation and migration and inhibits apoptosis; promotes tumorigenicity in vivo. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-139-5p to regulate PIEZO1 and activate FAK/Akt signaling. |
| Biological pathway or process: |
PI3K/AKT (promotes); proliferation (promotes); migration (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Survival Analysis; Transfection; CCK8; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
High circZNF800 levels are associated with lower survival rate and predict poor prognosis in GBM patients. |
| Description: |
circZNF800 (hsa_circ_0082096), derived from ZNF800 and enriched in GSLC exosomes, is up-regulated in GBM and mainly localized in the cytoplasm. It promotes GBM proliferation and migration and inhibits apoptosis by sponging miR-139-5p to increase PIEZO1 and activate downstream FAK/Akt signaling, and high circZNF800 predicts poorer survival. |
| Confidence score: |
0.8699 |