| Expression pattern: |
DN |
| Associated gene: |
EGFR, p38, cyclin D1, E-cadherin |
| Associated microRNA: |
- |
| Biological function: |
Overexpression of circ_4302 inhibited proliferation and migration of HUVECs, arrested cells at the G0/G1 stage, and promoted adhesion under an HCC microenvironment. |
| Molecular mechanism: |
circ_4302 overexpression decreased p-EGFR, p-p38 and cyclin D1 expression, while enhancing E-cadherin expression. |
| Biological pathway or process: |
EGFR (inhibits); MAPK (inhibits); proliferation (inhibits); migration (inhibits); cell cycle (inhibits); other pathway/process (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RT-qPCR; RNA-seq; Transfection; MTT; Transwell Assay; Cell Cycle Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
May provide new targets for HCC therapy; tissue validation and correlation with clinical characteristics remain to be analyzed. |
| Description: |
circ_4302 was one of the most significantly downregulated circRNAs in HUVECs exposed to an HCC microenvironment. Its overexpression reduced endothelial cell viability and migration, induced G0/G1 arrest, enhanced E-cadherin expression, and suppressed p-EGFR/p-p38 and cyclin D1, indicating a regulatory role in endothelial functions relevant to HCC progression. |
| Confidence score: |
0.5067 |