circRNA basic information
circBase ID: hsa_circ_0001727
Name: hsa_circ_ZKSCAN1
Synonym: circ_ZKSCAN1 / circZKSCAN1
Host Gene: ZKSCAN1
Genomic location(hg19): chr7:99621041-99621930:+
Genomic location(hg38): chr7:100023418-100024307:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues; matched adjacent normal tissues; xenograft tumor tissues
Cell lines:

A549; H1299; H460; H1650; NCI-2228; HBE
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: FAM83A, JNK, p38, ERK
Associated microRNA: miR-330-5p
Biological function: Circ-ZKSCAN1 promotes NSCLC progression by enhancing tumor cell proliferation, colony formation, migration, invasion and xenograft tumor growth, while inhibiting apoptosis; high expression is associated with larger tumor size, advanced clinical stage and poor prognosis.
Molecular mechanism: Circ-ZKSCAN1 functions as a ceRNA/miRNA sponge for miR-330-5p, thereby relieving miR-330-5p-mediated repression of FAM83A; FAM83A elevation feeds back to promote circ-ZKSCAN1 expression and the circ-ZKSCAN1/miR-330-5p/FAM83A loop inhibits MAPK/ERK signaling.
Biological pathway or process:

MAPK (inhibits); ERK (inhibits); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes); other pathway/process (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Annexin V/PI Flow Cytometry; In Vivo Animal Model; IHC (Immunohistochemistry); RNA Pull-Down; Luciferase Reporter Assay; Western Blot; Survival Analysis; Bioinformatics Analysis
Clinical significance:

Circ-ZKSCAN1 is significantly up-regulated in NSCLC and is associated with malignant characteristics, poor prognosis, tumor size and clinical stage; it may serve as a potential biomarker and therapeutic target.
Description:

Circ-ZKSCAN1 (hsa_circ_0001727), derived from ZKSCAN1, is up-regulated in NSCLC tissues and cell lines and high expression is linked to larger tumors, advanced clinical stage and poorer survival. Functionally, it promotes NSCLC proliferation, migration, invasion and xenograft tumor growth and suppresses apoptosis. Mechanistically, circ-ZKSCAN1 sponges miR-330-5p to increase FAM83A expression, forming a feedback loop that inhibits MAPK/ERK signaling and promotes NSCLC progression.
Confidence score:

0.7794
Other information
Title:

Circ-ZKSCAN1 regulates FAM83A expression and inactivates MAPK signaling by targeting miR-330-5p to promote non-small cell lung cancer progression.

Journal: Translational lung cancer research
Published: 2019
PubMed ID: 32010565
Study type:

combined biological and clinical study
Data availability: -
Code availability: -