| Expression pattern: |
UP |
| Associated gene: |
ANXA2, TFEB, ATG13, ULK1, AGO2 |
| Associated microRNA: |
miR-224-5p |
| Biological function: |
promotes autophagy, proliferation, migration, invasion, colony formation, angiogenesis, and metastasis; inhibits apoptosis; promotes malignant progression in TNBC |
| Molecular mechanism: |
circEGFR binds ANXA2 to separate the ANXA2-TFEB complex and promotes TFEB nuclear translocation; circEGFR acts as a ceRNA sponging miR-224-5p to upregulate ATG13 and ULK1, forming a miR-224-5p/ATG13/ULK1 feedback loop to enhance autophagy |
| Biological pathway or process: |
autophagy (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); angiogenesis (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; Bioinformatics Analysis; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; Western Blot; IF (Immunofluorescence); RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Co-IP; CCK8; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Tube Formation Assay; ELISA; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining |
| Clinical significance: |
circEGFR was significantly upregulated in TNBC and was positively associated with lymph node metastasis; circEGFR in plasma-derived exosomes was upregulated in breast cancer patients compared with healthy people; potential diagnosis biomarker and therapeutic target for TNBC |
| Description: |
circEGFR (hsa_circ_0080222), an EGFR-derived cytoplasmic circRNA enriched in exosomes, is upregulated in TNBC and plasma exosomes from breast cancer patients. It promotes TNBC autophagy and malignant behaviors by binding ANXA2 to enable TFEB nuclear translocation and by acting as a ceRNA for miR-224-5p to upregulate ATG13/ULK1. |
| Confidence score: |
0.8861 |