circRNA basic information
circBase ID: hsa_circ_0087855
Name: hsa_circ_RAD23B
Synonym: circRAD23B
Host Gene: RAD23B
Genomic location(hg19): chr9:110062421-110068928:+
Genomic location(hg38): chr9:107300140-107306647:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008170
MONDO name: ovarian cancer
Disease details: ovarian cancer / OC
Disease DO ID:
 2394
Disease MeSH ID:
 D010051
Disease NCIt ID:
 C7431
Disease ICD11 ID:
 685124533
Disease OMIM ID:
 167000
Species: Human
Species details: Homo sapiens
Tissue specimen:

OC tissues / ovarian tumour tissue
Cell lines:

Ovcar3; HO8910; A2780; HEY
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: YBX1
Associated microRNA: miR-1287-5p
Biological function: promotes proliferation; reduces sensitivity to carboplatin (carboplatin resistance); promotes tumorigenesis
Molecular mechanism: ceRNA mechanism: circRAD23B sponges miR-1287-5p to upregulate YBX1
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); drug resistance (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA-seq; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; EdU Staining; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); IF (Immunofluorescence); H&E Staining; Survival Analysis
Clinical significance:

CircRAD23B was significantly increased in the carboplatin-resistant group and negatively correlated with the overall survival of patients with OC.
Description:

CircRAD23B (hsa_circ_0087855), derived from RAD23B, is upregulated in ovarian cancer, particularly in carboplatin-resistant tissues, and is mainly localized in the cytoplasm. It promotes proliferation and carboplatin resistance by sponging miR-1287-5p to relieve repression of YBX1, thereby increasing YBX1 expression and enhancing tumor growth and chemoresistance in cells, patient-derived organoids, and xenograft models.
Confidence score:

0.8751
Other information
Title:

CircRAD23B promotes proliferation and carboplatin resistance in ovarian cancer cell lines and organoids.

Journal: Cancer cell international
Published: 2024
PubMed ID: 38273320
Study type:

combined biological and clinical study
Data availability: The online version contains supplementary material available at 10.1186/s12935-024-03228-1.
Code availability: -