| Expression pattern: |
UP |
| Associated gene: |
ALKBH5, YTHDF2 |
| Associated microRNA: |
miR-486-3p, miR-409-3p |
| Biological function: |
associated with increased Th17% and increased autophagy level in CD4+T cells in RA |
| Molecular mechanism: |
hypomethylated (reduced m6A) circFOXK2; ALKBH5 regulates m6A level and circFOXK2 expression; predicted ceRNA binding to miR-486-3p and miR-409-3p |
| Biological pathway or process: |
autophagy (promotes); immune regulation (other); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
Microarray; MeRIP / MeRIP-seq; RT-qPCR; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis; Transfection; Western Blot |
| Clinical significance: |
circFOXK2 shows diagnostic value for distinguishing new-onset RA from HC and from revisiting RA (ROC AUC 0.631 and 0.706) |
| Description: |
In peripheral blood CD4+ T cells from new-onset RA patients, circFOXK2 is up-regulated while its m6A-methylated level is decreased. circFOXK2 level correlates positively with Th17% and autophagy, and ALKBH5 modulates circFOXK2 m6A level and expression; bioinformatics predicts circFOXK2 may sponge miR-486-3p/miR-409-3p and has modest diagnostic value by ROC analysis. |
| Confidence score: |
0.7039 |