| Expression pattern: |
DN |
| Associated gene: |
p-AKT, p-mTOR, AKT, mTOR, Bcl-2, Bax, caspase-3, caspase-9 |
| Associated microRNA: |
- |
| Biological function: |
hsa_circ_0001649 inhibits RB cell growth and proliferation, promotes cell apoptosis, suppresses xenograft tumor growth, and acts as a tumor suppressor in RB. |
| Molecular mechanism: |
hsa_circ_0001649 regulates RB cell proliferation and apoptosis partly through negative regulation of AKT/mTOR signaling, with altered p-AKT and p-mTOR expression and apoptosis-related proteins Bcl-2, Bax, caspase-3 and caspase-9. |
| Biological pathway or process: |
PI3K/AKT/mTOR (inhibits); apoptosis (promotes); proliferation (inhibits); other pathway/process (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Cohort Study; Survival Analysis |
| Clinical significance: |
Downregulation of hsa_circ_0001649 is associated with larger tumor size and advanced IIRC stage, predicts worse 5-year overall survival, and hsa_circ_0001649 may be a prognostic biomarker and therapeutic target for RB. |
| Description: |
hsa_circ_0001649 is downregulated in retinoblastoma tissues and cell lines, and reduced expression is associated with aggressive clinicopathologic features and poor overall survival. Functionally, hsa_circ_0001649 suppresses RB cell proliferation and xenograft growth while promoting apoptosis, partly through inhibition of AKT/mTOR signaling. |
| Confidence score: |
0.6736 |