| Expression pattern: |
UP |
| Associated gene: |
UCK2 |
| Associated microRNA: |
miR-149-5p |
| Biological function: |
promotes HCC cell proliferation, colony formation, migration and invasion; inhibits apoptosis; promotes tumor growth in vivo |
| Molecular mechanism: |
circUCK2 acts as a ceRNA by sponging miR-149-5p to upregulate UCK2 |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RT-qPCR; circRNA-seq; RNase R Treatment; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; ROC Analysis; Transfection; EdU Staining; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; In Vivo Animal Model; RNA-seq |
| Clinical significance: |
circUCK2 was increased in serum samples from HCC patients and ROC curve analysis suggested diagnostic value (AUC = 0.77) |
| Description: |
circUCK2 is up-regulated in HCC tissues, cells, and patient serum. It promotes malignant phenotypes (proliferation, migration, invasion/EMT and tumor growth) and suppresses apoptosis by sponging miR-149-5p to upregulate UCK2. |
| Confidence score: |
0.8654 |