| Expression pattern: |
UP |
| Associated gene: |
AIFM2, GPX4, Ago2 |
| Associated microRNA: |
miR-6085, miR-6805 |
| Biological function: |
Promotes HCC progression and proliferation; inhibits ferroptosis. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge (miR-6805 and/or miR-6085), competing with AIFM2 and GPX4 for miRNA binding to increase AIFM2 and GPX4 expression and suppress ferroptosis. |
| Biological pathway or process: |
proliferation (promotes); ferroptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Transfection; CCK8; Transwell Assay; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
May represent a novel diagnostic marker and therapeutic target for HCC. |
| Description: |
circ0060467 is upregulated in HCC tissues and cell lines and promotes tumor growth. Mechanistically, it acts as a ceRNA/miRNA sponge to regulate AIFM2 and GPX4, thereby inhibiting ferroptosis and facilitating HCC progression. It is proposed as a potential diagnostic marker and therapeutic target for HCC. |
| Confidence score: |
0.8432 |