| Expression pattern: |
UP |
| Associated gene: |
E2F7, WDR66, BMI1, IGF2BP1, Ago2 |
| Associated microRNA: |
miR-653-5p |
| Biological function: |
Promotes proliferation, migration, invasion, EMT, tumor growth, liver metastasis, stemness and chemoresistance; inhibits 5-Fu-induced apoptosis. |
| Molecular mechanism: |
Acts as a miRNA sponge (ceRNA) for miR-653-5p to regulate E2F7, leading to transcriptional induction of WDR66; recruits IGF2BP1 to stabilize BMI1 mRNA. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); stemness (promotes); chemoresistance (promotes); apoptosis (inhibits); ceRNA regulation (promotes); mRNA stability (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; IF (Immunofluorescence); Western Blot; In Vivo Animal Model; H&E Staining; Survival Analysis; Bioinformatics Analysis; ChIP / ChIP-seq |
| Clinical significance: |
High circPOFUT1 level was positively correlated with TNM stage and associated with poor overall survival in CRC. |
| Description: |
circPOFUT1 (hsa_circ_0059774) is up-regulated in colorectal cancer and promotes proliferation, EMT, metastasis, stemness and 5-Fu chemoresistance. It functions as a ceRNA by sponging miR-653-5p to enhance E2F7 and induce WDR66, and it also recruits IGF2BP1 to stabilize BMI1 mRNA. High circPOFUT1 expression correlates with advanced TNM stage and poor overall survival. |
| Confidence score: |
0.8839 |