circRNA basic information
circBase ID: hsa_circ_0005273
Name: hsa_circ_PTK2
Synonym: -
Host Gene: PTK2
Genomic location(hg19): chr8:141710989-141716304:-
Genomic location(hg38): chr8:140700890-140706205:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005081
MONDO name: preeclampsia
Disease details: preeclampsia / PE
Disease DO ID:
10591
Disease MeSH ID:
D011225
Disease NCIt ID:
C85021
Disease ICD11 ID:
229121159
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

placental tissues

Cell lines:

HTR-8/SVneo; HTR8/SVneo

In vivo animal model:

-

circRNA-disease information
Expression pattern:
DN
Associated gene: WNT7B, Ago2
Associated microRNA: miR-619
Biological function: circPTK2 promotes trophoblast cell proliferation, migration, invasion and glucose metabolism; circPTK2 knockdown inhibits HTR8/SVneo cell proliferation, invasion, glucose uptake and lactate production.
Molecular mechanism: circPTK2 acts as a ceRNA sponge for miR-619 and regulates WNT7B expression through the circPTK2/miR-619/WNT7B axis.
Biological pathway or process:

Wnt/beta-catenin (promotes); proliferation (promotes); migration (promotes); invasion (promotes); glycolysis (promotes); ceRNA regulation (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Transwell Assay; Western Blot

Clinical significance:

circPTK2 has potential as a diagnostic biomarker and therapeutic target for EOPE.

Description:

circPTK2 is down-regulated in placental tissues from PE/EOPE patients. Functionally, circPTK2 promotes trophoblast proliferation, migration, invasion and glucose metabolism, while its knockdown suppresses these processes. Mechanistically, circPTK2 acts as a miR-619 sponge to regulate WNT7B expression, supporting its potential as a biomarker and therapeutic target for EOPE.

Confidence score:

0.725

Other information
Title:

circPTK2 promotes proliferation, migration and invasion of trophoblast cells through the miR-619/WNT7B pathway in preeclampsia.

Journal: Molecular and cellular biochemistry
Published: 2023
PubMed ID: 36913151
Study type:

combined biological and clinical study

Data availability: available within the article and its supplementary materials
Code availability: -