| Expression pattern: |
DN |
| Associated gene: |
pyruvate carboxylase |
| Associated microRNA: |
miR-181c-5p |
| Biological function: |
Modulates T cell function by inhibiting pyruvate carboxylase activity, decreasing extracellular glucose levels, suppressing miR-181c-5p, and increasing IL-2 secretion; promotes Jurkat cell activation and proliferation upon stimulation. |
| Molecular mechanism: |
IFN-alpha suppresses circ-CAMTA1 expression in Jurkat cells; circ-CAMTA1 physically binds pyruvate carboxylase (PC) and inhibits its enzymatic activity, affecting glucose metabolism; circ-CAMTA1 overexpression decreases miR-181c-5p, relieving translational inhibition of IL-2 and increasing IL-2 secretion. |
| Biological pathway or process: |
immune regulation (promotes); ceRNA regulation (other); other pathway/process (other); proliferation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; Transfection; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; ELISA; Flow Cytometry（Non-apoptosis/cycle）; MTT; Bioinformatics Analysis |
| Clinical significance: |
circ-CAMTA1 was significantly reduced in T cells from patients with SLE and its lower levels were associated with higher disease activity (SLEDAI-2K). |
| Description: |
circ-CAMTA1 is down-regulated in SLE patient T cells and inversely associated with disease activity. Mechanistically, circ-CAMTA1 binds and inhibits pyruvate carboxylase, impacts glucose metabolism, and suppresses miR-181c-5p leading to increased IL-2 secretion, thereby modulating T cell function. |
| Confidence score: |
0.7204 |