| Expression pattern: |
UP |
| Associated gene: |
HK2, LDHA |
| Associated microRNA: |
miR-577 |
| Biological function: |
Promotes OS cell proliferation, invasion and glycolysis, and inhibits apoptosis; promotes tumor growth in vivo. |
| Molecular mechanism: |
Acts as a miRNA sponge (ceRNA): circ_0000376 sponges miR-577, thereby regulating HK2 and LDHA expression and glycolysis-related malignant phenotypes in osteosarcoma. |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); apoptosis (inhibits); glycolysis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Microarray; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
Potential therapeutic target for osteosarcoma. |
| Description: |
circ_0000376 is upregulated in osteosarcoma and promotes proliferation, invasion, glycolysis (Warburg effect) while inhibiting apoptosis. Mechanistically, it sponges miR-577, releasing repression of HK2 and LDHA, thereby driving glycolysis and tumor growth in vitro and in a U2OS xenograft model. |
| Confidence score: |
0.7421 |