| Expression pattern: |
UP |
| Associated gene: |
IGF2BP2, NUP160, ZEB1, METTL3 |
| Associated microRNA: |
miRNA-126-3p |
| Biological function: |
Suppresses autophagy and promotes malignant phenotypes including proliferation, migration, invasion, colony formation, EMT, tumor growth and metastasis in LUAD. |
| Molecular mechanism: |
m6A-modified circRAPGEF5 binds IGF2BP2 (m6A reader), facilitating IGF2BP2-mediated stabilization of NUP160 mRNA, leading to autophagy suppression and LUAD progression/metastasis. |
| Biological pathway or process: |
autophagy (inhibits); proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); metastasis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; FISH / smFISH; RNA Pull-Down; RIP (RNA Immunoprecipitation); MeRIP / MeRIP-seq; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Western Blot; IF (Immunofluorescence); Actinomycin D / DRB Stability Assay; In Vivo Animal Model; H&E Staining; Bioinformatics Analysis; Survival Analysis |
| Clinical significance: |
Serum exosomal circRAPGEF5 may serve as a marker for identifying LUAD tumors; Patients with high expression of circRAPGEF5 had shorter survival. |
| Description: |
circRAPGEF5 is up-regulated in LUAD and predominantly localizes in the cytoplasm. It binds the m6A reader IGF2BP2 and promotes IGF2BP2-dependent stabilization of NUP160 mRNA, suppressing autophagy and thereby enhancing LUAD proliferation, EMT, invasion, and metastasis, including in xenograft models. |
| Confidence score: |
0.8326 |