| Expression pattern: |
DN |
| Associated gene: |
GCNT4 |
| Associated microRNA: |
miR-485-3p |
| Biological function: |
inhibits proliferation, migration, invasion, and glucose metabolism (glycolysis) of gastric cancer cells; suppresses tumor growth in vivo |
| Molecular mechanism: |
ceRNA mechanism: circPRDM5 sponges miR-485-3p to regulate (increase) GCNT4 expression |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); glycolysis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; RNA Pull-Down; Transfection; Colony Formation Assay; EdU Staining; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
GC patients with low circPRDM5 expression had poor prognoses when compared with those with high circPRDM5 expression; circPRDM5 had a high diagnostic value in distinguishing GC patients from healthy controls. |
| Description: |
circPRDM5 is downregulated in gastric cancer and functions as a tumor suppressor by inhibiting proliferation, migration/invasion, and glycolysis. Mechanistically, it sponges miR-485-3p to upregulate GCNT4, and higher circPRDM5 expression is associated with better overall survival. |
| Confidence score: |
0.8593 |