| Expression pattern: |
UP |
| Associated gene: |
YTHDF3, FASN mRNA, eIF4A3 |
| Associated microRNA: |
- |
| Biological function: |
promotes lymph node metastasis; promotes proliferation; promotes migration and invasion; promotes EMT; shortens DFS |
| Molecular mechanism: |
circZFR binds the m6A reader YTHDF3 and promotes m6A-dependent translation of FASN by facilitating YTHDF3 recognition on FASN mRNA and recruiting the translation initiator eIF4A3. |
| Biological pathway or process: |
EMT (promotes); metastasis (promotes); proliferation (promotes); migration (promotes); invasion (promotes); m6A modification (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Cohort Study; Survival Analysis; Transfection; RNA Pull-Down; RIP (RNA Immunoprecipitation); Co-IP; MeRIP / MeRIP-seq; Western Blot; CCK8; EdU Staining; Transwell Assay; In Vivo Animal Model; H&E Staining; Bioinformatics Analysis |
| Clinical significance: |
high circZFR expression is associated with lymph node metastasis and shortened disease-free survival (DFS) in cervical cancer patients |
| Description: |
In cervical cancer, circZFR is up-regulated and is associated with lymph node metastasis and shorter DFS. It promotes proliferation, migration/invasion and EMT by binding the m6A reader YTHDF3 to enhance m6A-dependent translation of FASN mRNA via recruitment of eIF4A3. |
| Confidence score: |
0.805 |