| Expression pattern: |
UP |
| Associated gene: |
PRMT9, E2F6, CCT2, AGO2, Notch1, Hes 1 |
| Associated microRNA: |
miR-671-5p |
| Biological function: |
Promotes proliferation, migration, invasion, EMT and metastasis; inhibits apoptosis (its knockdown increases apoptosis). |
| Molecular mechanism: |
E2F6 regulates CCT2 promoter to control hsa_circ_001726 expression; hsa_circ_001726 acts as a ceRNA sponge for miR-671-5p to elevate PRMT9, activating Notch1/Hes1 signaling to drive EMT. |
| Biological pathway or process: |
Notch (promotes); EMT (promotes); metastasis (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Bioinformatics Analysis; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; EdU Staining; Wound Healing Assay; Transwell Assay; TUNEL; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; ChIP / ChIP-seq |
| Clinical significance: |
Higher expression of hsa_circ_001726 was closely associated with poor prognosis of HCC patients. |
| Description: |
hsa_circ_001726 is up-regulated in HCC tissues/cell lines and promotes HCC malignant phenotypes and metastasis. Mechanistically, E2F6 enhances its expression via the CCT2 promoter, and hsa_circ_001726 sponges miR-671-5p to increase PRMT9, activating Notch1/Hes1-driven EMT. |
| Confidence score: |
0.8355 |