| Expression pattern: |
UP |
| Associated gene: |
HMGB1, FOXO1, AGO2, BIM, AKT |
| Associated microRNA: |
miR-142-3p |
| Biological function: |
promotes proliferation, migration, invasion and metastasis; inhibits apoptosis |
| Molecular mechanism: |
Acts as a ceRNA sponge for miR-142-3p to upregulate HMGB1 and activate MAPK/ERK and NF-kappaB; binds FOXO1 and sequesters it in the cytoplasm leading to feedback AKT activation and reduced BIM transcription |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); MAPK (promotes); ERK (promotes); NF-kappaB (promotes); PI3K/AKT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; EdU Staining; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; ROC Analysis |
| Clinical significance: |
circHERC1 is higher in patient plasma and tumor tissues; shows diagnostic value by ROC (AUC 0.746); proposed as potential prognostic biomarker and therapeutic target |
| Description: |
circHERC1 is up-regulated in NSCLC patient plasma/tissues and promotes malignant phenotypes. It enhances migration/invasion via the ceRNA axis circHERC1-miR-142-3p-HMGB1, activating MAPK/ERK and NF-kappaB signaling, and promotes proliferation while inhibiting apoptosis by binding FOXO1 and retaining it in the cytoplasm to feedback-activate AKT and affect BIM transcription. |
| Confidence score: |
0.868 |