circRNA basic information
circBase ID: hsa_circ_0085533
Name: hsa_circ_MYC
Synonym: circMYC / circ_0085533
Host Gene: c-Myc
Genomic location(hg19): chr8:128748314-128748869:+
Genomic location(hg38): chr8:127736068-127736623:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005105
MONDO name: melanoma
Disease details: melanoma
Disease DO ID:
 1909
Disease MeSH ID:
 D008545
Disease NCIt ID:
 C3224
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

human melanoma tissues; matched adjacent nontumor tissues
Cell lines:

Mel-CV; Mel-RM
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: LDHA, c-Myc, SRSF1
Associated microRNA: miR-1236
Biological function: circMYC promotes melanoma cell proliferation, viability, glycolysis, extracellular lactate production, and LDHA expression/activity.
Molecular mechanism: circMYC acts as a molecular sponge for miR-1236, relieving miR-1236-mediated repression of LDHA 3′ UTR and increasing LDHA; circMYC production is positively regulated by the c-Myc-SRSF1 axis.
Biological pathway or process:

proliferation (promotes); glycolysis (promotes); ceRNA regulation (other)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; RNA Pull-Down; Luciferase Reporter Assay; Western Blot; Northern Blot; Bioinformatics Analysis
Clinical significance:

circMYC has potential as a biomarker for melanoma diagnosis.
Description:

In melanoma, human circMYC (hsa_circ_0085533) is up-regulated in tumor tissues and promotes melanoma cell proliferation, viability, glycolysis, lactate production, and LDHA expression. Mechanistically, circMYC sponges miR-1236 to relieve repression of LDHA, while c-Myc and SRSF1 positively regulate circMYC production. The study suggests circMYC may serve as a diagnostic biomarker for malignant melanoma.
Confidence score:

0.8271
Other information
Title:

CircMYC Regulates Glycolysis and Cell Proliferation in Melanoma.

Journal: Cell biochemistry and biophysics
Published: 2020
PubMed ID: 31811601
Study type:

combined biological and clinical study
Data availability: -
Code availability: -