| Expression pattern: |
UP |
| Associated gene: |
NUDT21, MAPK14 (p38), STAT3 |
| Associated microRNA: |
hsa-miR-153-5p |
| Biological function: |
promotes proliferation; promotes migration; increases sensitivity to oxidative stress (H2O2); promotes p38 phosphorylation; promotes STAT3 phosphorylation |
| Molecular mechanism: |
Likely ceRNA/miRNA sponge mechanism: circBARD1 binds hsa-miR-153-5p and is associated with activation of p38 (MAPK14) signaling; circBARD1 expression is regulated by the RBP NUDT21. |
| Biological pathway or process: |
p38 MAPK signaling (promotes); MAPK (promotes); proliferation (promotes); migration (promotes); oxidative stress (other); ceRNA regulation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Transfection; In Vivo Animal Model; Bioinformatics Analysis; Luciferase Reporter Assay; Western Blot; Transwell Assay; CCK8 |
| Clinical significance: |
Subtype-associated biomarker: overexpressed in TCF3::PBX1 patients and part of a circRNA signature that discriminates TCF3::PBX1 ALL from other subtypes. |
| Description: |
circBARD1 (hsa_circ_0001098) is overexpressed in TCF3::PBX1 childhood B-ALL, is mainly cytoplasmic, and promotes leukemogenic phenotypes (proliferation and migration) in 697 cells. It is regulated by the RBP NUDT21 and is proposed to activate p38/MAPK signaling via hsa-miR-153-5p, with reduced p38 and STAT3 phosphorylation upon circBARD1 knockdown. |
| Confidence score: |
0.6157 |