circRNA basic information
circBase ID: hsa_circ_0067971
Name: hsa_circ_FNDC3B
Synonym: circFNDC3B
Host Gene: FNDC3B
Genomic location(hg19): chr3:171830241-171830380:+
Genomic location(hg38): chr3:172112451-172112590:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma / OS
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

OS tissues; adjacent tissues; OS tumor tissues
Cell lines:

U2OS; Saos-2; HOS; MG63; 143B; hFOB1.19
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: RBM47, IGF2BP1, FNDC3B mRNA
Associated microRNA: -
Biological function: suppresses osteosarcoma proliferation, migration, invasion and tumor growth
Molecular mechanism: RBM47 binds flanking introns to facilitate circFNDC3B biogenesis, reducing FNDC3B mRNA; circFNDC3B competitively binds IGF2BP1 to reduce FNDC3B mRNA stability
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); mRNA stability (inhibits); other pathway/process (other)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Actinomycin D / DRB Stability Assay; Transfection; CCK8; EdU Staining; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis
Clinical significance:

may be biomarkers for indicating disease progression and prognosis of OS patients
Description:

circFNDC3B is down-regulated in osteosarcoma and functions as a tumor suppressor, inhibiting OS cell proliferation, migration, invasion and xenograft tumor growth. Mechanistically, RBM47 promotes circFNDC3B biogenesis via binding flanking introns, and circFNDC3B competitively binds IGF2BP1 to reduce FNDC3B mRNA stability, contributing to a circular FNDC3B/FNDC3B mRNA imbalance.
Confidence score:

0.8396
Other information
Title:

A RBM47 and IGF2BP1 mediated circular FNDC3B-FNDC3B mRNA imbalance is involved in the malignant processes of osteosarcoma.

Journal: Cancer cell international
Published: 2023
PubMed ID: 38129874
Study type:

combined biological and clinical study
Data availability: The datasets used in the current study are available from the corresponding author upon request.
Code availability: -