| Expression pattern: |
UP |
| Associated gene: |
SLC7A11, Ago2 |
| Associated microRNA: |
miR-527 |
| Biological function: |
promotes DDP resistance and suppresses ferroptosis in OSCC cells |
| Molecular mechanism: |
acts as a molecular sponge for miR-527 to promote SLC7A11 expression, thereby inhibiting ferroptosis and increasing cisplatin resistance |
| Biological pathway or process: |
ferroptosis (inhibits); chemoresistance (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; MTT; Western Blot; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
higher circ_0000140 expression is associated with DDP-resistant OSCC patient tumors |
| Description: |
circ_0000140 is up-regulated in DDP-resistant OSCC tumors and resistant cell lines, where it promotes cisplatin resistance by inhibiting ferroptosis. Mechanistically, it sponges miR-527 to relieve repression of SLC7A11, increasing SLC7A11 and reducing ferroptotic activity, thereby elevating DDP IC50. |
| Confidence score: |
0.788 |