circRNA basic information
circBase ID: hsa_circ_0007265
Name: hsa_circ_ARHGAP10
Synonym: circRNA ARHGAP10 / circARHGAP10
Host Gene: ARHGAP10
Genomic location(hg19): chr4:148860975-148887990:+
Genomic location(hg38): chr4:147939824-147966839:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005463
MONDO name: aortic valve calcification
Disease details: calcific aortic valve disease / CAVD
Disease DO ID:
 -
Disease MeSH ID:
 -
Disease NCIt ID:
 -
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

CAV leaflet samples / CAV leaflets; control non-CAV samples; calcific aortic valves; mouse aortic valves
Cell lines:

VICs; HEK-293T; COS7; VECs
In vivo animal model:

genetically engineered animal model; other disease animal model
circRNA-disease information
Expression pattern:
UP
Associated gene: RUNX2, AGO2
Associated microRNA: miR-335-3p
Biological function: promotes osteogenic differentiation and calcification of VICs
Molecular mechanism: ceRNA (miRNA sponge) for miR-335-3p to regulate RUNX2 expression
Biological pathway or process:

ceRNA regulation (promotes); osteogenic differentiation (promotes); calcification (promotes)
Detected method:
Q
M
Validation methods:

Microarray; RNase R Treatment; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; Western Blot; In Vivo Animal Model; H&E Staining; ELISA
Clinical significance:

potential diagnostic and therapeutic target for CAVD
Description:

circARHGAP10 (hsa_circ_0007265) is up-regulated in calcified aortic valve tissue and promotes VIC osteogenic differentiation/calcification. It functions as a ceRNA that sponges miR-335-3p, thereby increasing RUNX2 expression, with in vitro assays and ApoE-/- CAVD model validation.
Confidence score:

0.7978
Other information
Title:

CircRNA ARHGAP10 promotes osteogenic differentiation through the miR-335-3p/RUNX2 pathway in aortic valve calcification.

Journal: Journal of thoracic disease
Published: 2023
PubMed ID: 38090284
Study type:

combined biological and clinical study
Data availability: -
Code availability: -