circRNA basic information
circBase ID: hsa_circ_0004104
Name: hsa_circ_SPARC
Synonym: circ_0004104
Host Gene: SPARC
Genomic location(hg19): chr5:151043647-151049345:-
Genomic location(hg38): chr5:151664086-151669784:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tumor tissues; adjacent normal tissues; xenograft tumor tissues
Cell lines:

SW480; HCT116; FHC
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: SYPL1, SPARC, PCNA, Cyclin D1, E-cadherin, N-cadherin, Vimentin, Ki67
Associated microRNA: miR-493-5p
Biological function: circ_0004104 promotes CRC cell proliferation, migration, invasion, metastasis, EMT-like marker changes, and xenograft tumor growth, while inhibiting apoptosis.
Molecular mechanism: circ_0004104 functions as a ceRNA/miRNA sponge for miR-493-5p to upregulate SYPL1 expression.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); apoptosis (inhibits); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RNase R Treatment; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High circ_0004104 expression predicted a poor prognosis of CRC patients and circ_0004104 expression was associated with tumor metastasis.
Description:

circ_0004104 is upregulated in CRC tissues and cell lines, and high expression is associated with poor prognosis. It promotes malignant CRC progression by sponging miR-493-5p to increase SYPL1 expression, thereby enhancing proliferation, migration, invasion, EMT-like marker changes, and xenograft tumor growth while reducing apoptosis.
Confidence score:

0.8239
Other information
Title:

A Novel Circ_0004104/MiR-493-5p/SYPL1 Cascade Contributes to Colorectal Cancer Progression.

Journal: Journal of biochemical and molecular toxicology
Published: 2025
PubMed ID: 40117336
Study type:

combined biological and clinical study
Data availability: Data are available from the corresponding author upon reasonable request.
Code availability: -