| Expression pattern: |
UP |
| Associated gene: |
CST4, Ago2 |
| Associated microRNA: |
miR-134-5p |
| Biological function: |
promotes cell growth, invasion, migration and tumorigenesis in ESCC |
| Molecular mechanism: |
ceRNA sponging of miR-134-5p leading to increased CST4 expression; CST4-dependent oncogenic effects |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); tumorigenesis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Bioinformatics Analysis; RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Transfection; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; Clinical Sample Validation |
| Clinical significance: |
High circ_0023984 expression was associated with poor overall survival in ESCC patients. |
| Description: |
circ_0023984 is up-regulated in ESCC tissues/cell lines and acts as an oncogenic circRNA. It mainly localizes to the cytoplasm and sponges miR-134-5p, thereby de-repressing CST4 to promote ESCC proliferation, migration/invasion, and xenograft tumor growth; high expression correlates with worse overall survival. |
| Confidence score: |
0.8489 |