| Expression pattern: |
UP |
| Associated gene: |
KHDRBS3, ZO-1, occludin, claudin-1, Ago2 |
| Associated microRNA: |
miR-577 |
| Biological function: |
Maintains BTB integrity and decreases BTB permeability; modulates tight junction-related proteins and affects doxorubicin penetration leading to glioma cell apoptosis. |
| Molecular mechanism: |
KHDRBS3 binds cDENND4C to increase its stability; cDENND4C acts as a ceRNA sponge for miR-577, relieving miR-577 repression of ZO-1/occludin/claudin-1 and thereby regulating BTB permeability. |
| Biological pathway or process: |
ceRNA regulation (promotes); apoptosis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Actinomycin D / DRB Stability Assay; Transfection; Western Blot; IF (Immunofluorescence); Transwell Assay; Annexin V/PI Flow Cytometry; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
- |
| Description: |
cDENND4C is up-regulated in glioma-associated endothelial cells (GECs) and helps maintain BTB integrity by sustaining tight junction protein expression. Mechanistically, KHDRBS3 binds cDENND4C to increase its stability, and cDENND4C sponges miR-577 to relieve repression of ZO-1/occludin/claudin-1, thereby reducing BTB permeability and limiting doxorubicin penetration. |
| Confidence score: |
0.8107 |