| Expression pattern: |
UP |
| Associated gene: |
WNT1, beta-catenin, TCF4 |
| Associated microRNA: |
- |
| Biological function: |
circ_001569 promotes NSCLC cell proliferation; knockdown of circ_001569 inhibits proliferation and colony formation; high circ_001569 expression is associated with poor survival and aggressive clinicopathological features. |
| Molecular mechanism: |
circ_001569 promotes NSCLC cell proliferation by regulating the Wnt/beta-catenin pathway; circ_001569 knockdown reduces mRNA and protein expression levels of WNT1, beta-catenin and TCF4. |
| Biological pathway or process: |
Wnt/beta-catenin (promotes); proliferation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Western Blot; Survival Analysis |
| Clinical significance: |
Increased circ_001569 expression is associated with tumor differentiation, lymph node metastasis and TNM classification, and high expression predicts poorer survival outcome in NSCLC. |
| Description: |
circ_001569 is up-regulated in NSCLC tissues and cell lines and its high expression is associated with tumor differentiation, lymph node metastasis, TNM classification and poor survival. Functionally, circ_001569 promotes NSCLC cell proliferation, while siRNA-mediated knockdown suppresses proliferation and colony formation. Mechanistically, circ_001569 appears to activate the Wnt/beta-catenin pathway by maintaining WNT1, beta-catenin and TCF4 mRNA/protein expression. |
| Confidence score: |
0.6148 |