| Expression pattern: |
DN |
| Associated gene: |
ZNF652, AGO2 |
| Associated microRNA: |
miR-337-3p |
| Biological function: |
Suppresses osteosarcoma cell proliferation, migration and invasion; suppresses tumor growth in vivo |
| Molecular mechanism: |
Acts as a miRNA sponge (ceRNA) for miR-337-3p to promote ZNF652 expression |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
B
|
| Validation methods: |
RT-qPCR; Northern Blot; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Sanger Sequencing; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Western Blot; Survival Analysis |
| Clinical significance: |
down-regulation of circVRK1 in osteosarcoma tissues was related to poor outcome of patients; low expression of circVRK1 was associated with distant metastasis |
| Description: |
circVRK1 is down-regulated in osteosarcoma and functions as a tumor suppressor by inhibiting proliferation, migration, invasion, and xenograft growth. Mechanistically, it acts as a ceRNA sponging miR-337-3p to increase ZNF652 expression, and low circVRK1 expression is associated with worse prognosis. |
| Confidence score: |
0.8654 |