circRNA basic information
circBase ID: -
Name: hsa_circ_E2F3
Synonym: circ-E2F3
Host Gene: E2F3
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0002974
MONDO name: cervical cancer
Disease details: cervical cancer
Disease DO ID:
 4362
Disease MeSH ID:
 -
Disease NCIt ID:
 C9311
Disease ICD11 ID:
 1256072522
Disease OMIM ID:
 603956
Species: Human
Species details: Homo sapiens
Tissue specimen:

CC tissues; matched adjacent tissues; adjacent normal tissues; tumor tissues
Cell lines:

c-33A; HeLa; SiHa; CaSki; HcerEpic; 293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: STAT3, cyclin D1
Associated microRNA: miR-296-5p
Biological function: circ-E2F3 promotes cervical cancer cell proliferation, migration, invasion, tumor growth, tumorigenesis, metastasis, and angiogenesis; knockdown of circ-E2F3 inhibits these malignant phenotypes and induces G0/G1 cell-cycle arrest.
Molecular mechanism: circ-E2F3 acts as a miR-296-5p sponge, inhibits miR-296-5p, increases STAT3 expression and phosphorylation, promotes STAT3 nuclear translocation, and upregulates cyclin D1 expression to drive cervical cancer progression.
Biological pathway or process:

JAK/STAT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); angiogenesis (promotes); cell cycle (promotes); ceRNA regulation (promotes)
Detected method:
Q
B
Validation methods:

RT-qPCR; RNase R Treatment; Northern Blot; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Cell Cycle Assay; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis
Clinical significance:

High circ-E2F3 expression is correlated with clinicopathological features, including FIGO stage, tumor size, lymph node metastasis and myometrial invasion, and poor prognosis of cervical cancer patients; it may be a potential biomarker to evaluate cervical cancer progression.
Description:

circ-E2F3 is upregulated in cervical cancer tissues and cell lines and is associated with aggressive clinicopathological features and poor prognosis. Functionally, circ-E2F3 promotes cervical cancer proliferation, migration, invasion, tumor growth, metastasis, and angiogenesis. Mechanistically, circ-E2F3 inhibits miR-296-5p to activate STAT3 nuclear translocation and increase cyclin D1 expression.
Confidence score:

0.7989
Other information
Title:

Circ-E2F3 promotes cervical cancer progression by inhibiting microRNA-296-5p and increasing STAT3 nuclear translocation.

Journal: Annals of the New York Academy of Sciences
Published: 2022
PubMed ID: 34468993
Study type:

combined biological and clinical study
Data availability: Table S1. RT-qPCR primers used in the present study.
Code availability: -