circRNA basic information
circBase ID: hsa_circ_0068888
Name: hsa_circ_WHSC1
Synonym: circ_0068,888
Host Gene: WHSC1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005229
MONDO name: bacterial infectious disease with sepsis
Disease details: sepsis-associated acute kidney injury
Disease DO ID:
 0040085
Disease MeSH ID:
 D016470
Disease NCIt ID:
 -
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

plasma
Cell lines:

HK-2
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: -
Associated microRNA: microRNA-21e5p (miR-21e5p), microRNA-21-5p
Biological function: Protects against LPS-induced HK-2 cell injury by increasing cell viability and inhibiting inflammatory response, oxidative stress, and NF-kB pathway activation.
Molecular mechanism: hsa_circ_0068,888 acts as a sponge for miR-21e5p and negatively regulates miR-21e5p expression, thereby inhibiting LPS-induced changes in cell viability, inflammatory response, oxidative stress, and NF-kB signaling.
Biological pathway or process:

NF-kappaB (inhibits); inflammation (inhibits); other pathway/process (inhibits)
Detected method:
Q
Validation methods:

RT-qPCR; Transfection; MTT; ELISA; Western Blot; IF (Immunofluorescence); Luciferase Reporter Assay; Bioinformatics Analysis
Clinical significance:

Up-regulation of hsa_circ_0068,888 might be a potential strategy in treatment for sepsis-associated AKI.
Description:

hsa_circ_0068,888 is down-regulated in LPS-induced HK-2 cell injury and was previously reported as down-regulated in plasma from patients with sepsis-associated AKI. In vitro, hsa_circ_0068,888 overexpression protects HK-2 cells by improving viability and reducing inflammation, oxidative stress, and NF-kB activation. Mechanistically, it sponges miR-21e5p, and miR-21e5p overexpression reverses the protective effects of hsa_circ_0068,888.
Confidence score:

0.5607
Other information
Title:

Circular RNA circ_0068,888 protects against lipopolysaccharide-induced HK-2 cell injury via sponging microRNA-21-5p.

Journal: Biochemical and biophysical research communications
Published: 2021
PubMed ID: 33429194
Study type:

biological research
Data availability: The data that support the findings of this study are available from the corresponding author upon reasonable request.
Code availability: -