| Expression pattern: |
UP |
| Associated gene: |
IGF2BP1, ARHGDIB mRNA, ERK |
| Associated microRNA: |
- |
| Biological function: |
Promotes melanoma cell proliferation and represses differentiation; silencing induces G0/G1 arrest and differentiation and inhibits tumor growth in vivo. |
| Molecular mechanism: |
circSipa1l1 binds the RBP IGF2BP1, enhancing IGF2BP1-ARHGDIB mRNA interaction and stabilizing ARHGDIB mRNA, thereby activating ERK signaling to promote proliferation and inhibit differentiation. |
| Biological pathway or process: |
ERK (promotes); cell cycle (promotes); proliferation (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; ISH (In Situ Hybridization); Clinical Sample Validation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Western Blot; IF (Immunofluorescence); In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
Highly expressed in melanoma patient tissues; suggested as a potential therapeutic target for melanoma differentiation therapy. |
| Description: |
circSipa1l1 is highly expressed in melanoma tissues/cells and functions as an oncogenic circRNA. It binds IGF2BP1 to facilitate IGF2BP1-dependent stabilization of ARHGDIB mRNA, thereby activating ERK signaling to promote cell-cycle progression and inhibit melanoma differentiation; silencing circSipa1l1 induces differentiation and suppresses xenograft tumor growth. |
| Confidence score: |
0.8571 |