circRNA basic information
circBase ID: hsa_circ_0043216
Name: hsa_circ_ACACA
Synonym: circRNA acetyl-CoA carboxylase alpha
Host Gene: ACACA
Genomic location(hg19): chr17:35441926-35444374:-
Genomic location(hg38): chr17:37084991-37087439:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer / CRC
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tissues; adjacent normal tissues; xenografted tumor tissues; lung tissues
Cell lines:

HT29; SW480; HCT116; RKO; SW620; NCM460
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: HDAC3, p53, HMGCS1, HMGCR, ACAT2, MVK, SREBP2, ABCA1, Ago2
Associated microRNA: miR-193a-3p, miR-193b-3p
Biological function: Promotes CRC cell proliferation, migration, invasion, tumor growth and lung metastasis; inhibits apoptosis; activates the MVA pathway.
Molecular mechanism: circACACA functions as a ceRNA sponge for miR-193a-3p and miR-193b-3p to enhance HDAC3 expression; HDAC3 inhibits p53 acetylation and transcriptional activity, thereby activating the MVA pathway and promoting CRC progression.
Biological pathway or process:

other pathway/process (promotes); p53 signaling (inhibits); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); ceRNA regulation (other); lipid metabolism (promotes)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; ChIP / ChIP-seq; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High circACACA expression is associated with larger tumor size, advanced TNM stage, tumor metastasis, lymph node metastasis, and shorter overall survival; circACACA is proposed as a prognostic marker and therapeutic target for CRC.
Description:

circACACA is upregulated in colorectal cancer tissues and cell lines and is associated with advanced clinicopathological features and poor overall survival. Functionally, it promotes CRC proliferation, migration, invasion, tumor growth and metastasis while suppressing apoptosis. Mechanistically, circACACA sponges miR-193a-3p and miR-193b-3p to increase HDAC3, reduce p53 acetylation/transcriptional activity, and activate the MVA pathway.
Confidence score:

0.8912
Other information
Title:

Circular RNA ACACA negatively regulated p53-modulated mevalonate pathway to promote colorectal tumorigenesis via regulating miR-193a/b-3p/HDAC3 axis.

Journal: Molecular carcinogenesis
Published: 2023
PubMed ID: 36920044
Study type:

combined biological and clinical study
Data availability: All data collected and analyzed during the current study are available from the corresponding author on reasonable request.
Code availability: -