circRNA basic information
circBase ID: hsa_circ_0007534
Name: hsa_circ_DDX42
Synonym: circ_0007534
Host Gene: DDX42
Genomic location(hg19): chr17:61869771-61877977:+
Genomic location(hg38): chr17:63792411-63800617:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0019087
MONDO name: cholangiocarcinoma
Disease details: cholangiocarcinoma
Disease DO ID:
 4947
Disease MeSH ID:
 D018281
Disease NCIt ID:
 C4436
Disease ICD11 ID:
 2110597275
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues; corresponding adjacent tissues; normal bile duct tissues; lung; liver
Cell lines:

HIBEC; CCLP-1; QBC939; RBE; HuCCT1
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: DDX3X, DDX42, DDX42 mRNA
Associated microRNA: -
Biological function: Promotes cholangiocarcinoma cell proliferation, migration, invasion, tumor stemness, anti-anoikis ability, tumor growth, and liver and lung metastasis.
Molecular mechanism: circ_0007534 forms a complex with the RNA-binding protein DDX3X, enhances DDX42 mRNA stability, upregulates DDX42 expression, and establishes a positive feedback loop with parental gene DDX42.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); stemness (promotes); apoptosis (inhibits); mRNA stability (promotes); other pathway/process (promotes)
Detected method:
Q
Validation methods:

RNase R Treatment; RT-qPCR; Clinical Sample Validation; Transfection; EdU Staining; Transwell Assay; Western Blot; Annexin V/PI Flow Cytometry; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RNA Pull-Down; RIP (RNA Immunoprecipitation); Actinomycin D / DRB Stability Assay; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Survival Analysis; ROC Analysis; Bioinformatics Analysis
Clinical significance:

High circ_0007534 expression correlated with lymph node invasion, advanced TNM stage, poor prognosis, and had prognostic potential with ROC AUC 0.779.
Description:

circ_0007534 is up-regulated in cholangiocarcinoma tissues and cell lines and is associated with lymph node invasion, advanced TNM stage, and poor prognosis. Functionally, it promotes proliferation, migration, invasion, stemness, anoikis resistance, tumor growth, and metastasis. Mechanistically, cytoplasmic circ_0007534 binds the RNA-binding protein DDX3X to stabilize DDX42 mRNA, forming a circ_0007534-DDX3X-DDX42 positive feedback loop that drives CCA progression.
Confidence score:

0.8717
Other information
Title:

Circ_0007534 promotes cholangiocarcinoma stemness and resistance to anoikis through DDX3X-mediated positive feedback regulation of parental gene DDX42.

Journal: Cellular signalling
Published: 2024
PubMed ID: 38492624
Study type:

combined biological and clinical study
Data availability: Data will be made available on request; Supplementary data to this article can be found online at https://doi. org/10.1016/j.cellsig.2024.111141.
Code availability: -