| Expression pattern: |
DN |
| Associated gene: |
FUS, SIRT1 |
| Associated microRNA: |
- |
| Biological function: |
Inhibits vascular smooth muscle cell calcification and calcium deposition. |
| Molecular mechanism: |
circHIPK3 binds/recruits the RNA-binding protein FUS to stabilize SIRT1 mRNA, activating SIRT1/PGC-1alpha signaling and promoting MFN2 expression to alleviate VSMC calcification. |
| Biological pathway or process: |
other pathway/process (inhibits); vascular calcification (inhibits); mRNA stability (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RIP (RNA Immunoprecipitation); RNA Pull-Down; Actinomycin D / DRB Stability Assay; Transfection; Western Blot; ELISA |
| Clinical significance: |
circHIPK3 is down-regulated in AS serum and may be a potential therapeutic target for vascular calcification. |
| Description: |
circHIPK3 is down-regulated in AS serum and in a beta-GP-induced VSMC calcification model. Its overexpression inhibits ALP activity and calcium deposition by recruiting FUS to stabilize SIRT1 mRNA and activating the SIRT1/PGC-1alpha/MFN2 pathway, thereby alleviating vascular calcification. |
| Confidence score: |
0.6575 |