| Expression pattern: |
DN |
| Associated gene: |
NLK, EIF4A3 |
| Associated microRNA: |
miR-665 |
| Biological function: |
inhibits proliferation, migration, invasion and EMT; inhibits TAM recruitment; suppresses tumor growth in vivo |
| Molecular mechanism: |
ceRNA sponge of miR-665 to upregulate NLK and suppress Wnt/beta-catenin signaling; circRNA biogenesis suppressed by EIF4A3 binding flanking sequences; downstream NLK-STAT3-CCL2 affects macrophage recruitment |
| Biological pathway or process: |
Wnt/beta-catenin (inhibits); EMT (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits); macrophage polarization (inhibits); immune regulation (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; Microarray; Sanger Sequencing; RNase R Treatment; Back-Splice Junction PCR / divergent primers PCR; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; EdU Staining; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; ELISA; IHC (Immunohistochemistry); Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis; Co-IP |
| Clinical significance: |
low circ_0009092 expression was associated with poor overall survival (OS) and recurrence-free survival (RFS); circ_0009092 was an independent prognostic factor |
| Description: |
In colorectal cancer, circ_0009092 is down-regulated and acts as a tumor suppressor by inhibiting proliferation, migration, invasion and EMT, and by reducing TAM recruitment. It functions mainly as a ceRNA that sponges miR-665 to increase NLK and inhibit Wnt/beta-catenin signaling; EIF4A3 suppresses its biogenesis, and the downstream NLK/STAT3/CCL2 module influences macrophage recruitment. |
| Confidence score: |
0.8772 |