| Expression pattern: |
UP |
| Associated gene: |
YB-1, HIP1, AKT |
| Associated microRNA: |
miR-615-5p |
| Biological function: |
Promotes proliferation, migration and invasion and inhibits apoptosis in glioma cells. |
| Molecular mechanism: |
YB-1-regulated circSPECC1 acts as a ceRNA/miRNA sponge for miR-615-5p to upregulate HIP1 and activate HIP1/AKT signaling. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); PI3K/AKT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RIP (RNA Immunoprecipitation); RT-qPCR; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Wound Healing Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot |
| Clinical significance: |
circSPECC1 is associated with the prognosis of glioma patients. |
| Description: |
In human glioma cell lines, circSPECC1 is upregulated and promotes proliferation, migration and invasion while inhibiting apoptosis. Mechanistically, it is regulated by YB-1 and functions as a ceRNA to sponge miR-615-5p, thereby increasing HIP1 and activating AKT signaling. |
| Confidence score: |
0.6578 |