| Expression pattern: |
DN |
| Associated gene: |
Foxo3 mRNA, Foxo3P, Foxo3 protein |
| Associated microRNA: |
miR-22, miR-136*, miR-138, miR-149*, miR-433, miR-762, miR-3614-5p, miR-3622b-5p |
| Biological function: |
Suppresses tumor growth, cancer cell proliferation and survival; decreases cell viability; promotes apoptosis/cell death; inhibits blood vessel formation/angiogenesis and local invasion; increases Foxo3 expression. |
| Molecular mechanism: |
circ-Foxo3 functions as a miRNA sponge for eight miRNAs, competitively reducing miRNA binding to Foxo3 mRNA and thereby promoting Foxo3 translation and protein expression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (inhibits); apoptosis (promotes); angiogenesis (inhibits); invasion (inhibits); other pathway/process (inhibits) |
| Detected method: |
Q
B
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Northern Blot; RNA Pull-Down; Luciferase Reporter Assay; Transfection; Annexin V/PI Flow Cytometry; Flow Cytometry（Non-apoptosis/cycle）; TUNEL; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); IF (Immunofluorescence); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
In this study, circ-Foxo3 is a Foxo3-derived circular RNA that is lower in breast tumor/cancer contexts than in normal tissues or non-cancer cell lines. Ectopic circ-Foxo3 suppresses breast cancer cell proliferation/survival and xenograft tumor growth, promotes apoptosis, reduces CD34-associated vascularization, and inhibits local invasion. Mechanistically, circ-Foxo3 acts as a sponge for eight miRNAs, relieving repression of Foxo3 mRNA translation and increasing Foxo3 protein expression. |
| Confidence score: |
0.78 |