| Expression pattern: |
UP |
| Associated gene: |
hnRNPA2B1, PTEN, AKT, mTOR |
| Associated microRNA: |
- |
| Biological function: |
promotes atherosclerosis progression and plaque formation; promotes cell proliferation and migration; inhibits apoptosis |
| Molecular mechanism: |
circZBTB46 physically interacts with hnRNPA2B1 and inhibits its ubiquitination-mediated proteasomal degradation, and modulates the PTEN/AKT/mTOR signaling pathway |
| Biological pathway or process: |
AKT/mTOR (promotes); PI3K/AKT/mTOR (promotes); proliferation (promotes); migration (promotes); apoptosis (inhibits); ubiquitination (inhibits) |
| Detected method: |
Q
H
|
| Validation methods: |
RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Transwell Assay; Wound Healing Assay; Annexin V/PI Flow Cytometry; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; Co-IP; Bioinformatics Analysis; In Vivo Animal Model; H&E Staining; Oil Red O Staining; Mass Spectrometry Analysis; Silver Staining; IHC (Immunohistochemistry) |
| Clinical significance: |
circZBTB46 has the potential to be a diagnostic biomarker for CAD |
| Description: |
circZBTB46 is up-regulated in human atherosclerotic plaques/CAD contexts and promotes plaque progression. Functionally, circZBTB46 supports endothelial and smooth muscle cell proliferation and migration while suppressing apoptosis. Mechanistically, it binds hnRNPA2B1 to reduce its ubiquitination and proteasomal degradation and modulates the PTEN/AKT/mTOR signaling pathway. |
| Confidence score: |
0.811 |