| Expression pattern: |
UP |
| Associated gene: |
ErbB, PI3K |
| Associated microRNA: |
miR-326 |
| Biological function: |
promotes HCC cell proliferation and tumor growth |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-326, leading to increased ErbB and PI3K expression |
| Biological pathway or process: |
proliferation (promotes); PI3K/AKT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Actinomycin D / DRB Stability Assay; Transfection; CCK8; EdU Staining; Luciferase Reporter Assay; RNA Pull-Down; Western Blot; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
circPTN may act as a diagnostic biomarker and a target for HCC |
| Description: |
circPTN is up-regulated in HCC tumor tissues and HCC cell lines and promotes HCC cell proliferation. Mechanistically, it localizes mainly in the cytoplasm and functions as a ceRNA by sponging miR-326, thereby increasing ErbB and PI3K expression and enhancing tumor growth-related proliferation. It is proposed as a potential diagnostic biomarker and therapeutic target in HCC. |
| Confidence score: |
0.8173 |