circRNA basic information
circBase ID: hsa_circ_0020378
Name: -
Synonym: circ_0020378 / circ _0020378
Host Gene: -
Genomic location(hg19): chr10:128594022-128926028:+
Genomic location(hg38): chr10:126905453-127127764:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

osteosarcoma specimens; matched normal bone tissues
Cell lines:

hFOB1.19; HOS; Saos-2; SW1353
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: COL1A1, Ago2
Associated microRNA: miR-339-3p
Biological function: promotes osteosarcoma cell proliferation, migration, colony formation, and tumor growth in vivo
Molecular mechanism: ceRNA sponging of miR-339-3p to relieve repression of COL1A1
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Nuclear-Cytoplasmic Fractionation; RNase R Treatment; Clinical Sample Validation; Transfection; CCK8; Transwell Assay; Colony Formation Assay; In Vivo Animal Model; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; Bioinformatics Analysis
Clinical significance:

-
Description:

hsa_circ_0020378 (circ_0020378) is up-regulated in osteosarcoma tissues and cell lines and promotes proliferation, migration, colony formation, and xenograft tumor growth. Mechanistically, it functions as a ceRNA by sponging miR-339-3p, thereby increasing COL1A1 expression and driving osteosarcoma progression.
Confidence score:

0.7795
Other information
Title:

hsa_circ_0020378 regulating miR-339-3p/COL1A1 promotes osteosarcoma progression.

Journal: Cancer biology & therapy
Published: 2023
PubMed ID: 37978903
Study type:

combined biological and clinical study
Data availability: All data generated or analyzed during this study are included in this published article.
Code availability: -