| Expression pattern: |
UP |
| Associated gene: |
SLUG, FUS |
| Associated microRNA: |
- |
| Biological function: |
promotes CRC cell migration, invasion, EMT, and metastasis; stabilizes SLUG and inhibits its ubiquitination-mediated degradation |
| Molecular mechanism: |
circSKA3 is retained in CRC cell cytoplasm via a cellmotif element (AGAAC) and directly binds SLUG protein to inhibit SLUG ubiquitination and proteasomal degradation, promoting EMT and metastasis; FUS binds circSKA3 motif to promote circularization |
| Biological pathway or process: |
EMT (promotes); metastasis (promotes); migration (promotes); invasion (promotes); ubiquitination (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Transfection; Transwell Assay; CCK8; RIP (RNA Immunoprecipitation); RNA Pull-Down; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Survival Analysis; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
high level of circSKA3 was significantly correlated with poor prognosis in CRC patients; decreased in serum of CRC patients with AUC 0.9674 |
| Description: |
circSKA3 (hsa_circ_0000467) is upregulated in CRC tumor tissues and mainly localizes to the cytoplasm. It directly binds SLUG via a cellmotif element to inhibit SLUG ubiquitination/proteasomal degradation, thereby promoting EMT, migration/invasion, and metastasis; FUS promotes circSKA3 circularization by binding a cis-motif. |
| Confidence score: |
0.8829 |