circRNA basic information
circBase ID: hsa_circ_0003251
Name: hsa_circ_WNK1
Synonym: circWNK1
Host Gene: WNK1
Genomic location(hg19): chr12:1003727-1006847:+
Genomic location(hg38): chr12:894561-897681:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer / GC
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

GC tissues; adjacent nontumor tissues / adjacent normal tissues
Cell lines:

GES-1; AGS; HGC-27; MKN-28; MKN-45; NCI-N87; HEK293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: SMAD7, Smad2, Smad3, P21
Associated microRNA: miR-21-3p
Biological function: Inhibits proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) of gastric cancer cells; suppresses tumor growth and metastasis in vivo.
Molecular mechanism: Acts as a ceRNA/miRNA sponge for miR-21-3p to relieve repression of SMAD7, thereby inhibiting TGF-beta signaling.
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); EMT (inhibits); TGF-beta/SMAD (inhibits); ceRNA regulation (promotes)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Sanger Sequencing; divergent primers PCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; IF (Immunofluorescence); Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Clinical Sample Validation; Survival Analysis; ROC Analysis; Bioinformatics Analysis
Clinical significance:

Low circWNK1 expression is associated with worse overall survival; circWNK1 shows diagnostic potential (ROC AUC 0.714).
Description:

circWNK1 (hsa_circ_0003251), derived from WNK1, is down-regulated in gastric cancer tissues/cell lines and acts as a tumor suppressor. It sponges miR-21-3p to restore SMAD7 expression, thereby inhibiting TGF-beta/SMAD signaling, leading to reduced proliferation, migration, invasion, EMT, tumor growth and metastasis. Low circWNK1 expression is linked to worse overall survival and has diagnostic potential (AUC 0.714).
Confidence score:

0.8889
Other information
Title:

Circular RNA circWNK1 inhibits the progression of gastric cancer via regulating the miR-21-3p/SMAD7 axis.

Journal: Cancer science
Published: 2024
PubMed ID: 38287200
Study type:

combined biological and clinical study
Data availability: -
Code availability: -