| Expression pattern: |
UP |
| Associated gene: |
HDGF, HIF1AN, METTL3, YTHDC1, hnRNPA2/B1, EpCAM, PI3K P85, PI3K P110, AKT, mTORC1, GSK-3beta, C-MYC, SURVIVIN, pre-CDYL |
| Associated microRNA: |
miR-892a, miR-328-3p |
| Biological function: |
promotes early hepatocarcinogenesis by enhancing stem-like properties (tumor-initiating capacity), proliferation, spheroid formation and colony formation; promotes malignancy via exosome-mediated intercellular transfer |
| Molecular mechanism: |
m6A modification promotes Circ-CDYL biogenesis by enhancing back-splicing via YTHDC1 and promotes exosomal sorting via hnRNPA2/B1; exosomal Circ-CDYL activates HDGF-PI3K-AKT-mTOR and HIF1AN-NOTCH2 signaling in recipient cells; Circ-CDYL acts as a sponge of miR-892a and miR-328-3p to regulate HDGF and HIF1AN |
| Biological pathway or process: |
PI3K/AKT/mTOR (promotes); Notch (promotes); proliferation (promotes); stemness (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; RNase R Treatment; MeRIP / MeRIP-seq; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; In Vivo Animal Model; Flow Cytometry（Non-apoptosis/cycle）; Actinomycin D / DRB Stability Assay; Bioinformatics Analysis; ROC Analysis; Clinical Sample Validation; Western Blot; Microarray |
| Clinical significance: |
Plasma exosomal Circ-CDYL (and combined with EpCAM+ exosomes as LTi-Exos, and further combined with AFP) shows high diagnostic accuracy for early HCC (ROC AUC up to 0.90). |
| Description: |
Circ-CDYL is up-regulated in very early/early HCC and is enriched in EpCAM+ plasma exosomes (LTi-Exos). m6A modification (via METTL3) promotes its back-splicing/biogenesis through YTHDC1 and enhances its loading into exosomes through hnRNPA2/B1, enabling exosomal transfer that activates HDGF-PI3K-AKT-mTOR and HIF1AN-NOTCH2 signaling to promote stemness and tumor initiation. Exosomal Circ-CDYL (alone or combined with EpCAM+ exosomes and AFP) shows strong potential for early HCC diagnosis by ROC analysis. |
| Confidence score: |
0.8621 |